Cell Total Iron Colorimetric Assay Kit (E-BC-K880-M)
For research use only.
| Detection Principle | Iron (Fe) is one of the metal elements in organism and has important physiological functions. Ferrous ion (Fe2+) is a key element in heme and hemoglobin, and plays an important role in many biochemical reactions. In the presence of reductant, iron ions in samples can be reduced into Fe2+. Fe2+ bind to probe and form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly. |
| Synonyms | Fe |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (590-600 nm) |
| Research Area | Inorganic Ions , Ferroptosis |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.4 μmol/L |
| Detection Range | 0.4-50 μmol/L |
| Precision | inter-assay CV: 1.5% | intra-assay CV: 1.3% |
| Sample Volume | 80 μL(cell homogenate) |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| HepG2 Cell | 1 |
| molt-4 Cell | 1 |
| Jurkat Cell | 1 |
| HEL Cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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