Cell Total Iron Colorimetric Assay Kit (E-BC-K880-M)

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All	Size	Price	Qty
	96T	$ 300.00
	48T	$ 200.00

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Detection Principle	Iron is one of the metal elements in organism and has important physiological functions. Ferrous ion is a key element in heme and hemoglobin and plays an important role in many biochemical reactions. Under the action of reductant, iron ions in samples can be reduced into ferrous ions (Fe²⁺). The latter then bind to probe and form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly.
Synonyms	Fe
Sample Type	Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (590-600 nm, optimum wavelength: 593 nm）
Research Area	Inorganic Ions , Ferroptosis
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.4 μmol/L
Detection Range	0.4-50 μmol/L
Precision	inter-assay CV: 1.5% \| intra-assay CV: 1.3%
Sample Volume	80 μL(cell homogenate)
Assay Time	1 h 20 min

Cat.No.	Product Name	Sizes
E-BC-K028-M	Malondialdehyde (MDA) Colorimetric Assay Kit (Cell Samples)	500Assays
E-BC-K030-M	Reduced Glutathione (GSH) Colorimetric Assay Kit	96T , 48T
E-BC-K138-F	Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green)	96T
E-BC-K881-M	Cell Ferrous Iron Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
HepG2 Cell	1
molt-4 Cell	1
Jurkat Cell	1
HEL Cell	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.