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Citrate Synthase(CS) Activity Assay Kit

Cat.No.:E-BC-K178-M
Detection instrument: Microplate reader (402-422 nm, optimum wavelength: 412 nm)

Detection method: Colorimetric method

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Detection principle

Citrate synthetase (CS) catalyzes acetyl-CoA and oxaloacetate to produce limonyl-CoA, which can further produce CoA. The yellow product generated by this reaction has a characteristic absorption peak at 412 nm. The activity of CS can be calculated by measuring the change of absorbance value at 412 nm.

Performance characteristics

Synonyms	CS
Sample type	animal, plant tissue and cell
Sensitivity	2.09 U/L
Detection range	2.09–110 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 7.900%Average intra-assay CV: 5%
Other instruments required	Incubator, Centrifuge
Storage	-20℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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