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Copper (Cu) Colorimetric Assay Kit

Cat.No.:E-BC-K300-M
Detection instrument: Microplate reader(575-585 nm)

Detection method: Colorimetric method

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Detection principle

In acidic condition, the copper ion in the sample react with 3,5-DiBr-PAESA to form a purple complex which has a maximum absorption peak at 580 nm. And copper ion content can be calculated indirectly by measuring the OD value at 580 nm.

Performance characteristics

Synonyms	Cu
Sample type	Serum,plasma,tissue
Sensitivity	1.84 μmol/L
Detection range	1.84-60 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 3.100%Average intra-assay CV: 3%
Other instruments required	Micropipettor, Incubator
Storage	2-8℃
Valid period	12 months

Images

No significant difference was observed in hippocampus, frontal cortex and thalamus in mice subject to KA when treatment with DPA, compared with vehicle group.

N Yang et al investigate the seizure-induced neurotoxicity revealed by D-penicillamine. Copper (Cu) levels in mouse brain were determined using copper colorimetric assay kit (E-BC-K300-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.84-60 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Dog serum	1
Rat serum	1
Rabbit serum	1
Porcine serum	1

Note: The diluent is double distilled water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Cells (2022) IF: 7.666
Cuproptosis-Related MiR-21-5p/FDX1 Axis in Clear Cell Renal Cell Carcinoma and Its Potential Impact on Tumor Microenvironment

DOI: 10.3390/cells12010173

Sample: OSRC-2 cells
Antioxidants (2022) IF: 7.675
D-Penicillamine Reveals the Amelioration of Seizure-Induced Neuronal Injury via Inhibiting Aqp11-Dependent Ferroptosis

DOI: 10.3390/antiox11081602

PMID: 36009321
Sample: brain
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208
ATF3/SPI1/SLC31A1 Signaling Promotes Cuproptosis Induced by Advanced Glycosylation End Products in Diabetic Myocardial Injury

DOI: 10.3390/ijms24021667

Sample: serum,myocardial tissue
Molecular Oncology (2021) IF: 6.603
Elesclomol induces copper-dependent ferroptosis in colorectal cancer cells via degradation of ATP7A

DOI: 10.1002/1878-0261.13079

PMID: 34390123
Sample: SW480 cell,DLD-1 cell
Microorganisms (2021) IF: 4.128
Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant Saccharomyces cerevisiae

DOI: 10.3390/microorganisms9020233

PMID: 33498600
Sample: Cell culture medium
Frontiers in Neuroscience (2021) IF: 4.677
Imbalances in Copper or Zinc Concentrations Trigger Further Trace Metal Dyshomeostasis in Amyloid-Beta Producing Caenorhabditis elegans

DOI: 10.3389/fnins.2021.755475

PMID: 34707479

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