Copper (Cu) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    <
    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K300-M

      Size:
      • 48T
      • 96T
      Qty:
      - +
      Price: $120

      Detection method: Colorimetric method

      Detection instrument: Microplate reader

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      General information

      Detection significance

      Copper is essential to all living organisms as a trace dietary mineral because it is a key constituent of the respiratory enzyme complex cytochrome c oxidase. In molluscs and crustaceans, copper is a constituent of the blood pigment hemocyanin, replaced by the iron-complexed hemoglobin in fish and other vertebrates. In humans, copper is found mainly in the liver, muscle, and bone. The adult body contains between 1.4 and 2.1 mg of copper per kilogram of body weight.

      Copper is involved in many biological processes, including enzyme reaction, nucleic acid synthesis, antioxidant defense, iron metabolism and immune function. Copper deficiency can affect the metabolism of bone and cholesterol and cause cardiovascular disease. Excess copper is associated with the damage of lung, kidney and liver.

      Detection principle

      In acidic condition, the copper ion in the sample react with 3,5-DiBr-PAESA to form a purple complex which has a maximum absorption peak at 580 nm. And copper ion content can be calculated indirectly by measuring the OD value at 580 nm.

      The key point

      1.   Serum and plasma samples should be clarified and avoid hemolysis. Heparin is recommended as anticoagulant.

      2.   Carry the assay in a ventilated place.

      Operation procedures

      Plate set up

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      A

      A

      S1

      S1

      S9

      S9

      S17

      S17

      S25

      S25

      S33

      S33

      B

      B

      B

      S2

      S2

      S10

      S10

      S18

      S18

      S26

      S26

      S34

      S34

      C

      C

      C

      S3

      S3

      S11

      S11

      S19

      S19

      S27

      S27

      S35

      S35

      D

      D

      D

      S4

      S4

      S12

      S12

      S20

      S20

      S28

      S28

      S36

      S36

      E

      E

      E

      S5

      S5

      S13

      S13

      S21

      S21

      S29

      S29

      S37

      S37

      F

      F

      F

      S6'

      S6

      S14

      S14

      S22

      S22

      S30

      S30

      S38

      S38

      G

      G

      G

      S7

      S7

      S15

      S15

      S23

      S23

      S31

      S31

      S39

      S39

      H

      H

      H

      S8

      S8

      S16

      S16

      S24

      S24

      S32

      S32

      S40

      S40

                                                                                 [Note]: A-H, standard wells; S1-S40, sample wells.

      The dilution of standard curve

      Dilute 100 μmol/L Copper ion standard with double distilled water to a serial concentration. The recommended dilution gradient is as follows: 60, 50, 40, 30, 20, 10, 5, 0 μmol/L.

      Operation steps

      1)   Standard tube: Take 20 μL of Standard solution with different concentrations into the wells.

      Sample tube: Take 20 μL of tested Sample into the wells.

      2)   Add 300 μL of Chromogenic agent into each tube of Step 1.

      3)   Cover the plate with sealer and incubate at 37℃ for 5 min.

      4)   Measure the OD value at 580 nm with Microplate Reader. 

      Operation table

       

      Standard tube

      Sample tube

      Copper ion standard with different concentrations (μL)

      20

       

      Sample (μL)

       

      20

      Chromogenic agent (μL)

      300

      300

      Cover the plate with sealer and incubate at 37 for 5 min and measure the OD value at 580 nm with Microplate Reader.

      Performance characteristics

      Technical parameter

      Detection range 1.84-60 μmol/L Average inter-assay CV 3.1%
      Sensitivity 1.84 μmol/L Average intra-assay CV 3%
      Average recovery rate 105%

      Standard curve

      Dilute 100 μmol/L Copper ion standard with double distilled water to a serial concentration. The recommended dilution gradient is as follows: 60, 50, 40, 30, 20, 10, 5, 0 μmol/L. Then carry the assay according to the operation table.

      Plot the standard curve by using OD value of standard and correspondent concentration as y-axis and x-axis respectively. Create the standard curve with graph software (or EXCEL). The concentration of the sample can be calculated according to the formula based on the OD value of sample. The standard curve is: y= ax + b.

      Example analysis

      Detect human serum, rat serum, rabbit serum and porcine serum according to the protocol, the result is as follows:
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA

      Browsing History


        People Also Bought