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Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)

  • Cat.No.:E-BC-K188-M

  • Detection instrument: Microplate reader(510-520 nm,optimum wavelength: 515 nm)

To Purchase E-BC-K188-M

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  • 48T
Price: $240
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Detection principle

Creatinine (Cr) can be catalyzed by creatinase and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatinase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm.

Performance characteristics

Synonyms Cr
Sample type Serum,plasma,urine
Sensitivity 3.8 μmol/L
Detection range 20.45-800 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 3.700%Average intra-assay CV: 1.400%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl)
Storage 2-8℃
Valid period 12 months

Images

No significant change of creatinine was observed, the LCFG nanoparticles did not include nephrotoxicity.

X Zhang et al investigate the usage of H2O2 and O2 for cooperative chemodynamic/starvation cancer therapy. Creatinine (Cr) level in mouse blood was determined using creatinine colorimetric assay kit (E-BC-K188-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (38.2-800 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human urine

20-30

Human serum

1

Rat serum

1

Porcine serum

1

Note: The diluent is normal saline (0.9% NaCl).

Citations

  1. BIOMATERIALS (2021) IF: 12.479
    Cyclic reactions-mediated self-supply of H2O2 and O2 for cooperative chemodynamic/starvation cancer therapy

    DOI: 10.1016/j.biomaterials.2021.120987

    PMID: 34175561

    Sample: MDA-MB-231 cells
  2. Acta Biomaterialia (2021) IF: 8.9469
    An efficient and safe MUC1-dendritic cell-derived exosome conjugate vaccine elicits potent cellular and humoral immunity and tumor inhibition in vivo

    DOI: 10.1016/j.actbio.2021.10.041

    PMID: 34757230

    Sample: serum
  3. Acta Biomaterialia (2021) IF: 8.9469
    A solid lipid coated calcium peroxide nanocarrier enables combined cancer chemo/chemodynamic therapy with O2/H2O2 self-sufficiency

    DOI: 10.1016/j.actbio.2020.12.036

    Sample: Serum,Plasma
  4. Biomaterials Science (2022) IF: 7.59
    Transdermal delivery of allopurinol on acute hyperuricemic mice via polymer microneedles for regulation of serum uric acid levels

    DOI: 10.1039/D2BM01836E

    Sample: serum
  5. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

    DOI: 10.3390/ijms232315400

    Sample: plasma,urine
  6. PHYTOTHERAPY RESEARCH (2022) IF: 6.388
    Resveratrol reverses TGF-β1-mediated invasion and metastasis of breast cancer cells via the SIRT3/AMPK/autophagy signal axis

    DOI: 10.1002/ptr.7608

    PMID: 36086852

    Sample: serum
  7. Pharmaceutics (2023) IF: 6.525
    Ultrasonically Fabricated Beta-Carotene Nanoemulsion: Optimization, Characterization and Evaluation of Combinatorial Effect with Quercetin on Streptozotocin-Induced Diabetic Rat Model

    DOI: 10.3390/pharmaceutics15020574

    Sample: serum
  8. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    Capsaicin Prevents Contrast-Associated Acute Kidney Injury through Activation of Nrf2 in Mice

    DOI: 10.1155/2022/1763922

    PMID: 35615576

    Sample: serum
  9. CELL BIOLOGY AND TOXICOLOGY (2023) IF: 6.819
    Syringaresinol protects against diabetic nephropathy by inhibiting pyroptosis via NRF2-mediated antioxidant pathway

    DOI: 10.1007/s10565-023-09790-0

    Sample: urine
  10. JOURNAL OF ETHNOPHARMACOLOGY (2023) IF: 5.195
    Yishen Tongluo formula alleviates diabetic kidney disease through regulating Sirt6/TGF-β1/Smad2/3 pathway and promoting degradation of TGF-β1

    DOI: 10.1016/j.jep.2023.116243

    Sample: kidney
  11. Frontiers in Pharmacology (2022) IF: 5.811
    Stewed Rhubarb Decoction Ameliorates Adenine-Induced Chronic Renal Failure in Mice by Regulating Gut Microbiota Dysbiosis.

    DOI: 10.3389/fphar.2022.842720

    Sample: plasma
  12. Drug Design Development and Therapy (2021) IF: 4.162
    Total Glucosides of Paeony Inhibited Autophagy and Improved Acute Kidney Injury Induced by Ischemia-Reperfusion via the lncRNA TUG1/miR-29a/PTEN Axis

    DOI: 10.2147/DDDT.S286606

    PMID: 34079224

    Sample: Serum
  13. Drug Design Development and Therapy (2021) IF: 4.162
    Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

    DOI: 10.2147/DDDT.S326975

    PMID: 34703210

    Sample: serum
  14. Biomed Research International (2022) IF: 3.246
    Evaluation Value of Serum miR-4299 and miR-16-5p in Risk Stratification of Sepsis-Induced Acute Kidney Injury

    DOI: 10.1155/2022/5165892

    PMID: 35845963

    Sample: serum
  15. Dose-Response (2022) IF: 2.623
    Biochemical Investigation of Therapeutic Potentials of Plant-Based Bioactive Compounds as Stimulators of Glucagon like peptide-1 Secretion :

    DOI: 10.1177/15593258221114184

    Sample: serum
  16. SMALL RUMINANT RESEARCH (2022) IF: 1.611
    Meat production, feed and water efficiencies of selected South African sheep breeds

    DOI: 10.1016/j.smallrumres.2022.106746

    Sample: urine
  17. DRUG DEVELOPMENT RESEARCH (2019) IF: 1.742
    Montelukast modifies simvastatin‐induced myopathy and hepatotoxicity

    DOI: 10.1002/ddr.21581

    Sample: liver
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