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Diamine Oxidase (DAO) Activity Assay Kit

  • Cat.No.:E-BC-K524-M

  • Detection instrument: Microplate reader (450-470 nm, optimum wavelength: 460 nm)

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Price: $320

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Detection principle

Diamine oxidase can catalyse amine substances to produce hydrogen peroxide, and hydrogen peroxide can react with the chromogenic substance to produce chromogenic substance, which has a characteristic absorption peak at 460 nm. The activity of diamine oxidase can be calculated by measuring the change rate of absorbance per unit time.

Performance characteristics

Synonyms DAO
Sample type serum, plasma, urine, animal tissue and cell samples
Sensitivity 7.39 U/L
Detection range 7.39-134.15 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 60 min
Precision Average inter-assay CV: 7%Average intra-assay CV: 5%
Other instruments required Centrifuge, Incubator, Microplate reader
Storage 2-8℃
Valid period 12 months


Activity of DAO was significantly induced by ethanol. (**P<0.01)

L Zhao et al investigate the osteoporosis and altered gut microbiota induced by ethanol. Diamine oxidase (DAO) activity of rat serum was determined using DAO activity assay kit (E-BC-K524-M).


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