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Diamine Oxidase (DAO) Activity Assay Kit

Cat.No.:E-BC-K524-M
Detection instrument: Microplate reader (450-470 nm, optimum wavelength: 460 nm)

Detection method: Colorimetric method

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96T

Price: $320

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Detection principle

Diamine oxidase can catalyse amine substances to produce hydrogen peroxide, and hydrogen peroxide can react with the chromogenic substance to produce chromogenic substance, which has a characteristic absorption peak at 460 nm. The activity of diamine oxidase can be calculated by measuring the change rate of absorbance per unit time.

Performance characteristics

Synonyms	DAO
Sample type	serum, plasma, urine, animal tissue and cell samples
Sensitivity	7.39 U/L
Detection range	7.39-134.15 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 7%Average intra-assay CV: 5%
Other instruments required	Centrifuge, Incubator, Microplate reader
Storage	2-8℃
Valid period	12 months

Images

Activity of DAO was significantly induced by ethanol. (**P<0.01)

L Zhao et al investigate the osteoporosis and altered gut microbiota induced by ethanol. Diamine oxidase (DAO) activity of rat serum was determined using DAO activity assay kit (E-BC-K524-M).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

CELL PROLIFERATION (2022) IF: 6.831
Altered gut microbiota and metabolites profile are associated with reduced bone metabolism in ethanol-induced osteoporosis

DOI: 10.1111/cpr.13245

PMID: 35688648
Sample: serum

Reviews/Q&A

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Q&A

C**************aSubmitted [ Sep 19 2023 ]

Asked: To whom it may concern, I am preparing a diamine oxidase (DAO) activity detection assay and I have seen your website. I would like to know if the peroxide formed comes from histamine metabolism or it comes from another amine such as putrescine or cadaverine. I would also like to know if DAO activity can be also detected in food samples in this kit, instead serum, orine, plasma, animal tissue and cell samples. Best regards, Cristina.

adminSubmitted [ Sep 19 2023 ]

Answered: Hi Cristina, kindly note the substrate of this kit belongs to diamines. This kit does not differentiate between species, Elabscience metabolism kits are designed to detect all types of samples, including food samples.

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