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Diamine Oxidase (DAO) Activity Assay Kit

Cat.No.:E-BC-K524-M
Detection instrument: Microplate reader (450-470 nm, optimum wavelength: 460 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Diamine oxidase can catalyse amine substances to produce hydrogen peroxide, and hydrogen peroxide can react with the chromogenic substance to produce chromogenic substance, which has a characteristic absorption peak at 460 nm. The activity of diamine oxidase can be calculated by measuring the change rate of absorbance per unit time.

Performance characteristics

Synonyms	DAO
Sample type	serum, plasma, urine, animal tissue and cell samples
Sensitivity	7.39 U/L
Detection range	7.39-134.15 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 7%Average intra-assay CV: 5%
Other instruments required	Centrifuge, Incubator, Microplate reader
Storage	2-8℃
Valid period	12 months

Images

Activity of DAO was significantly induced by ethanol. (**P<0.01)

L Zhao et al investigate the osteoporosis and altered gut microbiota induced by ethanol. Diamine oxidase (DAO) activity of rat serum was determined using DAO activity assay kit (E-BC-K524-M).

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Collection and treatment of common samples for metabolism assay detection

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