D-Lactic Acid/Lactate(LA)Colorimetric Assay Kit (E-BC-K002-M)
For research use only.
| Detection Principle | Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated. |
| Synonyms | D-LA |
| Sample Type | Serum,Plasma,Animal tissue,Saliva sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (520-550 nm, optimum wavelength: 530 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 mmol/L |
| Detection Range | 0.06-8.0 mmol/L |
| Precision | inter-assay CV: 7.7% | intra-assay CV: 3.8% |
| Sample Volume | 5 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 1 |
| Human serum | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Mouse serum | 1 |
| Rabbit serum | 1 |
| 10% Rat kidney tissue homogenate | 2-3 |
| 10% Mouse brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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