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Elab Fluor® 488 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]

  • Cat.No.:E-AB-F1112L

  • Applications: FCM
  • Isotype: Rat IgM, κ
  • Host: Rat

To Purchase E-AB-F1112L

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  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $55
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Antigen Details

Alternate Names B220
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Product Details

Form Liquid
Clone No. RA3.3A 1/6.1(See Other Available Formats)
Host Rat
Isotype Rat IgM, κ
Isotype Control Elab Fluor® 488 Rat IgM, κ Isotype Control[RTK2118] [Product E-AB-F09772L]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Elab Fluor® 488
Elab Fluor® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F11120 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112A 25μg, 100μg Check More
Biotin Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112B 25μg, 100μg Check More
FITC Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112C 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112D 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112E 50Tests, 100Tests, 100Tests×2 Check More
PerCP Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112F 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112G 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112H 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112I 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112J 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] 50Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112UC 25μg, 100μg Check More
PE Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112UD 25μg, 100μg Check More
APC Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112UE 25μg, 100μg Check More
PerCP Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112UF 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112UG 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112UH 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112UI 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] E-AB-F1112UJ 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] 25μg, 100μg Check More
PE/Elab Fluor® 594 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1] 25μg, 100μg Check More

Citations

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