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EPI(Epinephrine/Adrenaline) ELISA Kit

Cat.No.:E-EL-0045
Reactivity: Universal

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes EPI in samples. No significant cross-reactivity or interference between EPI and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of EPI concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with EPI. During the reaction, EPI in the sample or standard competes with a fixed amount of EPI on the solid phase supporter for sites on the Biotinylated Detection Ab specific to EPI. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EPI in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level EPI were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level EPI were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	96.50	321.00	901.00	88.20	307.60	931.00
Standard deviation	6.30	13.50	36.00	4.90	13.50	37.20
CV (%)	6.53	4.21	4.00	5.56	4.39	4.00

Recovery

The recovery of EPI spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-101	93
EDTA plasma(n=8)	95-109	101
Cell culture media(n=8)	89-100	95

Linearity

Samples were spiked with high concentrations of EPI and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Epinephrine, adrenaline, adrenalin, 4, 5-β-trihydroxy-N-methylphenethylamine
Research Area	Cell Biology, Metabolism, Epigenetics and Nuclear Signal, Signal transduction, Neuroscience, Cardiovascular, Cancer, Biochemicals

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

CELL (2023) IF: 66.849
Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis

DOI: 10.1016/j.cell.2022.12.024

PMID: 36669474
Sample: Serum
Cellular & Molecular Immunology (2023) IF: 22.096
β-adrenoreceptor-triggered PKA activation negatively regulates the innate antiviral response

DOI: 10.1038/s41423-022-00967-x

PMID: 36600052
Sample: serum
ENVIRONMENTAL POLLUTION (2022) IF: 9.988
Neuroendocrine stress hormones associated with short-term exposure to nitrogen dioxide and fine particulate matter in individuals with and without chronic obstructive pulmonary disease: A panel study in Beijing, China

DOI: 10.1016/j.envpol.2022.119822

PMID: 35870527
Sample: serum
Computational and Structural Biotechnology Journal (2020) IF: 6.018
Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors

DOI: 10.1016/j.csbj.2020.11.009

PMID: 33304464
Sample: blood
Molecular Metabolism (2019) IF: 6.181
Metformin prevents the pathological browning of subcutaneous white adipose tissue

DOI: 10.1016/j.molmet.2019.08.011

Sample: plasma
Communications Biology (2022) IF: 6.548
Maternal anxiety affects embryo implantation via impairing adrenergic receptor signaling in decidual cells

DOI: 10.1038/s42003-022-03694-1

PMID: 35982177
Sample: serum,uteri
JOURNAL OF NEUROSCIENCE (2022) IF: 6.709
Uncoupling protein -1 modulates anxiety-like behavior in a temperature-dependent manner

DOI: 10.1523/JNEUROSCI.2509-21.2022

PMID: 36194650
Sample: serum
Journal of the International Society of Sports Nutrition (2020) IF: 5.068
Caffeine supplementation induces higher IL-6 and IL-10 plasma levels in response to a treadmill exercise test

DOI: 10.1186/s12970-020-00375-4

PMID: 32907591
Sample: Plasma
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2022) IF: 4.114
Protective effect of low-dose radiation on doxorubicin-induced brain injury in mice

DOI: 10.1016/j.abb.2022.109390

PMID: 36067878
Sample: brain tissue
ACS Omega (2022) IF: 4.132
Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion

DOI: 10.1021/acsomega.2c03619

PMID: 35967043
Sample: serum
ACTA DIABETOLOGICA (2022) IF: 4.28
Deep transcranial magnetic stimulation in combination with skin thermography in obesity: a window on sympathetic nervous system

DOI: 10.1007/s00592-022-01859-2

PMID: 35174415
Sample: blood
Molecular Brain (2023) IF: 4.399
Investigating the molecular mechanisms of delirium-like neuropsychiatric disorder induced by electromagnetic pulse based on bioinformatics analysis

DOI: 10.1186/s13041-023-00998-z

Sample: prefrontal cortex,hippocampus
ENDOCRINE (2018) IF: 3.179
High frequency deep transcranial magnetic stimulation acutely increases β-endorphins in obese humans

DOI: 10.1007/s12020-018-1791-1

Sample: blood
MEDIATORS OF INFLAMMATION (2015) IF: 3.236
Intranasal Dexmedetomidine on Stress Hormones, Inflammatory Markers, and Postoperative Analgesia after Functional Endoscopic Sinus Surgery

DOI: 10.1155/2015/939431

Sample: Serum
PLoS One (2017) IF: 3.24
Perivascular radiofrequency renal denervation lowers blood pressure and ameliorates cardiorenal fibrosis in spontaneously hypertensive rats

DOI: 10.1371/journal.pone.0176888

Sample: plasma
International Journal of Environmental Research and Public Health (2021) IF: 3.39
A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients

DOI: 10.3390/ijerph18094852

PMID: 34062830
Sample: Serum
JOURNAL OF FISH BIOLOGY (2022) IF: 2.051
Primary stress response and biochemical profile of Labeo rohita (Hamilton, 1822) experimentally parasitized with Argulus bengalensis (Ramakrishna, 1951)

DOI: 10.1111/jfb.15048

PMID: 35319798
Sample: plasma
Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
LianXia Formula Granule Attenuates Cardiac Sympathetic Remodeling in Rats with Myocardial Infarction via the NGF/TrKA/PI3K/AKT Signaling Pathway

DOI: 10.1155/2021/5536406

PMID: 34221073
Sample: plasma
AMERICAN JOURNAL OF HYPERTENSION (2016) IF: 2.689
A Novel Swine Model of Spontaneous Hypertension With Sympathetic Hyperactivity Responds Well to Renal Denervation

DOI: 10.1093/ajh/hpv066

Sample: Plasma,Urine
PLoS One (2018) IF: 2.766
Selective ablation of the ligament of Marshall reduces ischemia and reperfusion-induced ventricular arrhythmias

DOI: 10.1371/journal.pone.0203083

PMID: 30153281
Sample: Serum
JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2018) IF: 0.545
Stress response to ovariohysterectomy in rabbits: role of anaesthesia and surgery

DOI: 10.1080/01443615.2017.1400523

Sample: Serum,Plasma
SMALL RUMINANT RESEARCH (2018) IF: 0.974
Effects of stocking density on stress responses and meat quality characteristics of lambs transported for 45 minutes or 3 hours

DOI: 10.1016/j.smallrumres.2018.08.009

Sample: serum

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DOI: 10.1016/j.cell.2022.12.024
PMID: 36669474	Sample: Serum

DOI: 10.1038/s41423-022-00967-x
PMID: 36600052	Sample: serum

DOI: 10.1016/j.envpol.2022.119822
PMID: 35870527	Sample: serum

DOI: 10.1016/j.csbj.2020.11.009
PMID: 33304464	Sample: blood

DOI: 10.1038/s42003-022-03694-1
PMID: 35982177	Sample: serum,uteri

DOI: 10.1523/JNEUROSCI.2509-21.2022
PMID: 36194650	Sample: serum

DOI: 10.1186/s12970-020-00375-4
PMID: 32907591	Sample: Plasma

DOI: 10.1016/j.abb.2022.109390
PMID: 36067878	Sample: brain tissue

DOI: 10.1021/acsomega.2c03619
PMID: 35967043	Sample: serum

DOI: 10.1007/s00592-022-01859-2
PMID: 35174415	Sample: blood

DOI: 10.1186/s13041-023-00998-z
	Sample: prefrontal cortex,hippocampus

DOI: 10.1093/ajh/hpv066
	Sample: Plasma,Urine

DOI: 10.1080/01443615.2017.1400523
	Sample: Serum,Plasma

Cat. No	E-EL-0045
Species

DOI: 10.3390/ijerph18094852
PMID: 34062830	Sample: Serum

DOI: 10.1111/jfb.15048
PMID: 35319798	Sample: plasma

DOI: 10.1155/2021/5536406
PMID: 34221073	Sample: plasma

DOI: 10.1371/journal.pone.0203083
PMID: 30153281	Sample: Serum

EPI(Epinephrine/Adrenaline) ELISA Kit

To Purchase E-EL-0045

Product Details

Properties

Test Principle

Kit components & Storage

Technical Data

Precision

Recovery

Linearity

Target Information

Assay Procedures

Citations

CELL (2023) IF: 66.849

Cellular & Molecular Immunology (2023) IF: 22.096

ENVIRONMENTAL POLLUTION (2022) IF: 9.988

Computational and Structural Biotechnology Journal (2020) IF: 6.018

Molecular Metabolism (2019) IF: 6.181

Communications Biology (2022) IF: 6.548

JOURNAL OF NEUROSCIENCE (2022) IF: 6.709

Journal of the International Society of Sports Nutrition (2020) IF: 5.068

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2022) IF: 4.114

ACS Omega (2022) IF: 4.132

ACTA DIABETOLOGICA (2022) IF: 4.28

Molecular Brain (2023) IF: 4.399

ENDOCRINE (2018) IF: 3.179

MEDIATORS OF INFLAMMATION (2015) IF: 3.236

PLoS One (2017) IF: 3.24

International Journal of Environmental Research and Public Health (2021) IF: 3.39

JOURNAL OF FISH BIOLOGY (2022) IF: 2.051

Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63

AMERICAN JOURNAL OF HYPERTENSION (2016) IF: 2.689

PLoS One (2018) IF: 2.766

JOURNAL OF OBSTETRICS AND GYNAECOLOGY (2018) IF: 0.545

SMALL RUMINANT RESEARCH (2018) IF: 0.974

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