Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor^® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]

Elab Fluor^® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] out of stock

Uniprot : Q02242

Cat.No.:E-AB-F1131US
Applications: FCM
Isotype: Rat IgG2a, κ
Host: Rat

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Antigen Details

Alternate Names	PD-1, Programmed Death-1
Swissprot	Q02242
Gene ID	18566
References	1. Nishimura H, et al. 2001. Science 291:319 2. Agata Y, et al. 1996. Int. Immunol. 8:765 3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706 4. Barber DL, et al. 2006. Nature 439:682 5. Keir ME, et al. 2005. J. Immunol. 175:7372 6. Koehn BH. et al. 2015.

Product Details

Form	Liquid
Clone No.	29F.1A12(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	Elab Fluor^® Red 780 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833S]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® Red 780
	Elab Fluor^® Red 780 is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 770 nm (e.g., a 780/60 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
Biotin Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12]	25μg, 100μg	Check More

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