Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> FITC Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133C
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|Alternate Names||B7-H1, PD-L1,Programmed cell death ligand 1,B7 homolog 1,B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1|
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
|Clone No.||29E.2A3(See Other Available Formats)|
|Isotype||Mouse IgG2b, κ|
|Isotype Control||FITC Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812C]|
|Application||FCM (See the Application Description)|
|Storage Buffer||Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.|
|Recommended Use||Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.|
|Shipping||Biological ice pack at 4℃|
|Stability & Storage||Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
|FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).|
How to select the appropriate detection channel through the spectrogram?
|CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.|
|Related Protocols||Staining Cell Surface Targets for Flow Cytometry|
|AF/LE Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F11330||50μg, 500μg, 1mg||Check More|
|Purified Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133A||25μg, 100μg||Check More|
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|PE Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133D||20Tests, 100Tests, 100Tests×2||Check More|
|APC Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133E||20Tests, 100Tests, 100Tests×2||Check More|
|PE/Cyanine5 Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133G||20Tests, 100Tests, 100Tests×2||Check More|
|PE/Cyanine7 Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133H||20Tests, 100Tests, 100Tests×2||Check More|
|PerCP/Cyanine5.5 Anti-Human CD274/PD-L1 Antibody[29E.2A3] E-AB-F1133J||20Tests, 100Tests, 100Tests×2||Check More|
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