Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> FITC Anti-Human CD35 Antibody[E11] E-AB-F1062C
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|Alternate Names||Complement receptor type 1,CR1,C3b/C4b receptor,CD35,CR1,C3BR|
1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS.
2. Klickstein LB, et al. 1988. J. Exp. Med. 168:1699.
|Clone No.||E11(See Other Available Formats)|
|Isotype||Mouse IgG1, κ|
|Isotype Control||FITC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792C]|
|Application||FCM (See the Application Description)|
|Storage Buffer||Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.|
|Recommended Use||Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.|
|Shipping||Biological ice pack at 4℃|
|Stability & Storage||Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
|FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).|
How to select the appropriate detection channel through the spectrogram?
|CD35 is a type I single chain of glycoprotein, also known as C3b/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, and follicular dendritic cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. CD35 has also been reported to inhibit T-cell proliferation.|
|Related Protocols||Staining Cell Surface Targets for Flow Cytometry|
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