Click here to view cell apoptosis assay products
×

FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12]

Uniprot : Q02242
  • Cat.No.:E-AB-F1131UC

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1131UC

Size:
  • 25μg
  • 100μg
Price: $54
Qty:

Antigen Details

Alternate Names PD-1, Programmed Death-1
Swissprot Q02242
References
1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2010.

Product Details

Form Liquid
Clone No. 29F.1A12
Host Rat
Isotype Rat IgG2a, κ
Isotype Control FITC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833C]
Reactivity Mouse
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
Biotin Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] 25μg, 100μg Check More

Citations

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for 10 Test Free Trial FCM Antibody
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: