Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> FITC Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103C
Cat.No.:E-AB-F1103C
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Alternate Names | T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E |
Swissprot | P22646 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263. |
Form | Liquid |
Clone No. | 145-2C11(See Other Available Formats) |
Host | Armenian Hamster |
Isotype | Armenian Hamster IgG |
Isotype Control | FITC Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F09852C] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | FITC |
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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