Alternate Names	B220
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Product Details

Form	Liquid
Clone No.	RA3.3A 1/6.1(See Other Available Formats)
Host	Rat
Isotype	Rat IgM, κ
Isotype Control	FITC Rat IgM, κ Isotype Control[RTK2118] [Product E-AB-F09772C]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	FITC
	FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Fas^lpr and MRL-Fas^gld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
Biotin Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
PE Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
PE Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
APC Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
PerCP Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD45R/B220 Antibody[RA3.3A 1/6.1]	25μg, 100μg	Check More

Citations

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Oxidative Medicine and Cellular Longevity (2023) IF: 7.31

Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function

DOI: 10.1155/2023/2894695
	Sample: colonic lamina propria cell

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