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FITC Anti-Rat CD45 Antibody[OX-1]

  • Cat.No.:E-AB-F1227C

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1227C

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  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $42
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Antigen Details

Alternate Names Receptor-type tyrosine-protein phosphatase C,Leukocyte common antigen, LCA, T200, Ly-5,Ptprc
Swissprot P04157
References
3. Zheng Z, et al. 1995. J Cell Bio. 130(2):485

Product Details

Form Liquid
Clone No. OX-1(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control FITC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792C]
Reactivity Rat
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

  1. Redox Biology (2023) IF: 10.787
    Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats

    DOI: 10.1016/j.redox.2023.102649

    Sample: bone marrow mesenchymal stem cells (BMMSCs)
  2. Stem Cell Research & Therapy (2022) IF: 8.0790
    MiR-152-5p suppresses osteogenic differentiation of mandible mesenchymal stem cells by regulating ATG14-mediated autophagy

    DOI: 10.1186/s13287-022-03018-4

    PMID: 35883156

    Sample: MMSCs-M
  3. ARCHIVES OF ORAL BIOLOGY (2021) IF: 2.635
    The polypeptide OP3-4 induced osteogenic differentiation of bone marrow mesenchymal stem cells via protein kinase B/glycogen synthase kinase 3β/β-catenin pathway and promoted mandibular defect bone regeneration

    DOI: 10.1016/j.archoralbio.2021.105243

    PMID: 34416564

    Sample: BMSCs
  4. Journal of Thoracic Disease (2021) IF: 2.895
    Mesenchymal stem cells transfected with anti-miRNA-204-3p inhibit acute rejection after heart transplantation by targeting C-X-C motif chemokine receptor 4 (CXCR4) in vitro

    DOI: 10.21037/jtd-21-1293

    PMID: 34527345

    Sample: MSCs
  5. Journal of Thoracic Disease (2021) IF: 2.895
    Mesenchymal stem cells transfected with anti-miRNA-204-3p inhibit acute rejection after heart transplantation by targeting C-X-C motif chemokine receptor 4 (CXCR4) in vitro

    DOI: 10.21037/jtd-21-1293

    PMID: 34527345

    Sample: MSCs
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Verified Customer

Q****HSubmitted [ Jul 03 2019 ]

  • Application:FCM
  • Species:Rat
  • Sample:blood
  • Primary antibody:Undiluted

    30minutes

  • Temperature:4℃

  • Description:This antibody is used well
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