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Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)

Cat.No.:E-BC-K234-M
Detection instrument: Microplate reader(500-510 nm)

Detection method: Colorimetric method

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Detection principle

Glucose oxidase can catalyze the oxidation of glucose to gluconic acid to produce hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide and oxidizes pigment sources to form colored substances. Measure the OD value at 505 nm and glucose content can be calculated indirectly.

Performance characteristics

Synonyms	Glu
Sample type	Serum,plasma,whole blood
Sensitivity	0.04 mmol/L
Detection range	0.04-30 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 2.300%Average intra-assay CV: 1.900%
Other instruments required	Vortex mixer, Micropipettor, Incubator
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.04-30 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
Rat serum	1
Human plasma	1

Note: The diluent is normal saline (0.9% NaCl) .

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2023) IF: 7.088
PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism

DOI: 10.1016/j.ejmech.2023.115166
Cell Communication and Signaling (2022) IF: 7.525
Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth

DOI: 10.1186/s12964-022-00882-8

PMID: 35842652
Sample: SGC-996 cell,EH-GB1 cell,GBC-SD cell
FOOD RESEARCH INTERNATIONAL (2022) IF: 6.475
Acrylamide induced glucose metabolism disorder in rats involves gut microbiota dysbiosis and changed bile acids metabolism

DOI: 10.1016/j.foodres.2022.111405

PMID: 35761659
Sample: serum
CANCER SCIENCE (2022) IF: 6.518
circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

DOI: 10.1111/cas.15351

Sample: AGS cell,HGC-27 cell
Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy

DOI: 10.1155/2022/4608914

PMID: 35498125
Sample: H9c2 cell
Pharmaceuticals (2022) IF: 5.215
Lycopene Modulates Oxidative Stress and Inflammation in Hypercholesterolemic Rats

DOI: 10.3390/ph15111420

Sample: serum
Frontiers in Endocrinology (2021) IF: 5.555
Metabolic Consequences of Neuronal HIF1α-Deficiency in Mediobasal Hypothalamus in Mice

DOI: 10.3389/fendo.2021.668193

PMID: 34733235
Sample: serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Canagliflozin Modulates Hypoxia-Induced Metastasis, Angiogenesis and Glycolysis by Decreasing HIF-1α Protein Synthesis via AKT/mTOR Pathway

DOI: 10.3390/ijms222413336

Sample: HepG2 cell
Microorganisms (2021) IF: 4.128
Candida albicans Hexokinase 2 Challenges the Saccharomyces cerevisiae Moonlight Protein Model

DOI: 10.3390/microorganisms9040848

PMID: 33920979
Sample: Cell sample
EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
Dapagliflozin attenuates steatosis in livers of high-fat diet-induced mice and oleic acid-treated L02 cells via regulating AMPK/mTOR pathway

DOI: 10.1016/j.ejphar.2021.174304

Sample: L02 cells
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
Isoliquiritigenin ameliorates caerulein‐induced chronic pancreatitis by inhibiting the activation of PSCs and pancreatic infiltration of macrophages

DOI: 10.1111/jcmm.15498

Sample: Serum
International Journal of Environmental Research and Public Health (2022) IF: 4.614
Effects of an Exercise Program Combining Aerobic and Resistance Training on Protein Expressions of Neurotrophic Factors in Obese Rats Injected with Beta-Amyloid

DOI: 10.3390/ijerph19137921

PMID: 35805580
Sample: plasma
REPRODUCTION (2020) IF: 3.206
Orlistat reverses intratesticular lactate transport decline and infertility in male obese rats

DOI: 10.1530/REP-20-0381

PMID: 33112813
Sample: testis
APPETITE (2021) IF: 3.868
Acute and short-term effects of Lactobacillus paracasei subsp. paracasei 431 and inulin intake on appetite control and dietary intake: A two-phases randomized, double blind, placebo-controlled study

DOI: 10.1016/j.appet.2021.105855

PMID: 34915104
Sample: serum
THERIOGENOLOGY (2020) IF: 2.094
Diet-induced obesity is associated with altered expression of sperm motility-related genes and testicular post-translational modifications in a mouse model

DOI: 10.1016/j.theriogenology.2020.09.023

PMID: 32980686
Sample: Serum
Biomedical Engineering Online (2021) IF: 2.819
Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression

DOI: 10.1186/s12938-021-00903-6

PMID: 34217312
Sample: Huh-7 cells
Cancer Management and Research (2020) IF: 2.886
Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis

DOI: 10.2147/CMAR.S242135

PMID: 32669871
Sample: Cell culture supernatant
Cancer Management and Research (2020) IF: 2.886
CircSEC31A Promotes the Malignant Progression of Non-Small Cell Lung Cancer Through Regulating SEC31A Expression via Sponging miR-376a

DOI: 10.2147/CMAR.S280124

PMID: 33204164
Sample: Cell sample
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2019) IF: 2.914
Isolation, characterization, and therapeutic activity of bergenin from marlberry (Ardisia colorata Roxb.) leaf on diabetic testicular complications in Wistar albino rats

DOI: 10.1007/s11356-019-04139-9

PMID: 30648235
Sample: Serum
BIOSCIENCE REPORTS (2020) IF: 2.942
Dexmedetomidine alleviated neuropathic pain in dorsal root ganglion neurons by inhibition of anaerobic glycolysis activity and enhancement of ROS tolerance

DOI: 10.1042/BSR20191994

PMID: 32285913
Sample: Cell sample

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Reviews/Q&A

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Reviews
Q&A

M**********dSubmitted [ Aug 12 2022 ]

Asked: Is it possible to measure the glucose concentration in juice?

adminSubmitted [ Aug 12 2022 ]

Answered: Yes, it is. There is no species distinction for samples for metabolism assays. But it's better to make a sample control for your samples especially for the first time.

D********dSubmitted [ Aug 01 2022 ]

Asked: How long can I keep the working solution?

adminSubmitted [ Aug 01 2022 ]

Answered: I't better to finishing using the working solution as soon as possible. If it's not available to use it immediately, the working solution must be protect from light at 2-8 degree, and no more than 24 hours.

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