Glutathione Peroxidase (GSH-PX) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K096-S

      Size:
      • 50 Assays
      • 100 Assays
      Qty:
      - +
      Price: $180

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      General information

      Detection significance

      Glutathione Peroxidase (GSH-PX) is an important enzyme that catalyzes decomposition of hydrogen peroxide. GSH specifically catalyze the reaction between GSH and hydrogen peroxide, protecting cell membrane structure and keeping membrane function integrity. Se-cysteine is the active center of the GSH-PX. Determination of GSH-PX activity in organism can be an indicator of selenium level as Se is essential section of GSH-PX.

      Detection principle

      Glutathione Peroxidase (GSH-PX) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-PX, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

      The key point

      If the GSH-PX activity is calculated by protein concentration, the protein concentration of the sample needs to be determined separately (E-BC-K318-M, E-BC-K168-S, E-BC-K165-S).

      Operation procedures

      Performance characteristics

      Technical parameter

      Detection range 12.65-387 U Average inter-assay CV 9.3%
      Sensitivity 12.65 U Average intra-assay CV 4.9%
      Average recovery rate 105%

      Citations

      1. Publication: Kim J, Choung S Y. Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups[J]. Molecular Vision, 2017, 23: 638-648.
        Species: Universal
        Sample Type: Tissue homogenate
      2. Publication: Siboto A, Sibiya N, Khathi A, et al. The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers[J]. Journal of Diabetes Research, 2018, 2018.
        Species: Rat
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      • Reviews (0)
      • Q&A (1)
      Q

      T****************gSubmitted [ Apr 23 2019 ]

      Asked: We don\'t have 412 nm of colorimetric spectro filter in my lab, we only have the 415 nm. And so, is it ok if we use 415nm? would it effect the accuracy of the measurement significantly? Could give me the contract of distributor in Thailand. Thank you.

      Reply

      A

      adminSubmitted [ Apr 23 2019 ]

      Answered: Dear Thapanee, Thanks for your message. It is ok to use 412nm to test the kit. Pls contact with the distributor to have the kit. Pacific Science Co.,Ltd. 62,64 Soi Charansanitwong 49/1 Charansanitwong rd. Bangbumru Bangplad Bangkok 10700 THAILAND Tel : +662-4330068-9 ext. 212 Email : pacscien@ksc.th.com Any questions, pls feel free to ask. Best regards,

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