Glutathione Peroxidase (GSH-Px) Activity Assay Kit
Price: $ 260
Price: $ 180
- Sample Type: serum;plasma;cells;cell culture supernatant;tissue
- Detection Instrument: Spectrophotometer(412 nm)
- Reasearch Areas: Oxidative stress;Plant Physiology Series
For research use only. Order now, ship in 3 days
Detection Principle | Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH. | ||||||||||
Synonyms | GSH-PX | ||||||||||
Sample Type | serum,plasma,cells,cell culture supernatant,tissue | ||||||||||
Sensitivity | 12.65 U | ||||||||||
Detection Range | 12.65-387 U | ||||||||||
Detection Method | Colorimetric method | ||||||||||
Assay Time | 70 min | ||||||||||
Precision | inter-assay CV: 9.3% | intra-assay CV: 4.9% | ||||||||||
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) | ||||||||||
Storage | This product can be stored at 2~8°C for 12 months with shading light. | ||||||||||
Valid Period | 12 months | ||||||||||
Images | |||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor. |
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