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Detection method: Colorimetric method
Detection instrument: Spectrophotometer(412 nm)
|Sample type||Serum,plasma,cells,cell culture supernatant,tissue|
|Detection range||12.65-387 U|
|Detection method||Colorimetric method|
|Assay type||Enzyme Activity|
|Assay time||70 min|
|Precision||Average inter-assay CV: 9.3%Average intra-assay CV: 4.9%|
|Other instruments required||Micropipettor, Incubator, Vortex mixer, Centrifuge|
|Other reagents required||Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)|
|Valid period||6 months|
The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 20%~60% (the optimal inhibition ratio is the range of 45%~55%.) before formal experiment.
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
F**oSubmitted [ Dec 19 2020 ]
Asked: I confused about the blank tube. If I have double beam spektro, what solution do i use for zeroing? And what blank for the standard & sample ?
adminSubmitted [ Dec 19 2020 ]
Answered: Hi Faro, Thanks for your message. Could you let us know which country you are from, so that I could forward to our local sales representative to contact you ASAP. Best regards
T****************gSubmitted [ Apr 23 2019 ]
Asked: We don\'t have 412 nm of colorimetric spectro filter in my lab, we only have the 415 nm. And so, is it ok if we use 415nm? would it effect the accuracy of the measurement significantly? Could give me the contract of distributor in Thailand. Thank you.
adminSubmitted [ Apr 23 2019 ]
Answered: Dear Thapanee, Thanks for your message. It is ok to use 412nm to test the kit. Pls contact with the distributor to have the kit. Pacific Science Co.,Ltd. 62,64 Soi Charansanitwong 49/1 Charansanitwong rd. Bangbumru Bangplad Bangkok 10700 THAILAND Tel : +662-4330068-9 ext. 212 Email : firstname.lastname@example.org Any questions, pls feel free to ask. Best regards,