Glutathione Peroxidase (GSH-Px) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K096-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $260
      Can detect 48 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(412 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Detection principle

      Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.

      Performance characteristics

      Synonyms GSH-PX
      Sample type Serum,plasma,cells,cell culture supernatant,tissue
      Sensitivity 12.65 U
      Detection range 12.65-387 U
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 70 min
      Precision Average inter-assay CV: 9.3%Average intra-assay CV: 4.9%
      Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      The optimal sampling volume are different for different species, also are different for different sample type. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the inhibition ratio is 20%~60% (the optimal inhibition ratio is the range of 45%~55%.) before formal experiment.

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

      Citations

      1. Supplementation of Short-Chain Fatty Acid, Sodium Propionate, in Patients on Maintenance Hemodialysis: Beneficial Effects on Inflammatory Parameters and Gut-Derived Uremic Toxins, A Pilot Study (PLAN Study)
        IF: 5.688
        Journal: Journal of Clinical Medicine(2018)
        DOI: 10.3390/jcm7100315
        PMID: 30274359
        Products: E-BC-K096-S 
        Sample type: Serum
        Reactivity: Human
      2. Intraocular pressure-lowering effects of imidazo [1, 2-a]-and pyrimido [1, 2-a] benzimidazole compounds in rats with dexamethasone-induced ocular hypertension
        IF: 3.17
        Journal: European Journal of Pharmacology(2019)
        DOI: 10.1016/j.ejphar.2019.01.059
        PMID: 30716317
        Products: E-BC-K020-M E-BC-K031-S E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      3. Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups
        IF: 2.174
        Journal: Molecular Vision(2017)
        DOI: 99999999
        PMID: 28943754
        Products: E-BC-K096-S 
        Sample type: Cataractous lenses
        Reactivity: Rat
      4. The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers.
        IF: 3.04
        Journal: Journal of Diabetes Research(2018)
        DOI: 10.1155/2018/7341242
        PMID: 30009183
        Products: E-BC-K096-S E-BC-K020-M 
        Sample type: Tissue homogenate
        Reactivity: Rat
      5. Pinus Densiflora Bark Extract Prevents Selenite-induced Cataract Formation In The Lens Of Sprague Dawley Rat Pups.
        IF: 2.174
        Journal: Molecular Vision(2017)
        PMID: 28943754
        Products: E-BC-K096-S 
        Sample type: Tissue homogenate
        Reactivity: Rat
      6. The Effect of Memantine Hydrochloride on Cisplatin-induced Toxicity with Reference to Hepatic and Haematological Alterations in Mice
        IF: 0.24
        Journal: Latin American Journal of Pharmacy(2019)
        DOI: 99999999
        PMID: 99999999
        Products: E-BC-K020-M E-BC-K025-S E-BC-K029-S E-BC-K030-M E-BC-K031-S E-BC-K096-S 
        Sample type: Serum,Tissue homogenate
        Reactivity: Mouse
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      • Reviews (0)
      • Q&A (2)
      Q

      F**oSubmitted [ Dec 19 2020 ]

      Asked: I confused about the blank tube. If I have double beam spektro, what solution do i use for zeroing? And what blank for the standard & sample ?

      Reply

      A

      adminSubmitted [ Dec 19 2020 ]

      Answered: Hi Faro, Thanks for your message. Could you let us know which country you are from, so that I could forward to our local sales representative to contact you ASAP. Best regards

      Q

      T****************gSubmitted [ Apr 23 2019 ]

      Asked: We don\'t have 412 nm of colorimetric spectro filter in my lab, we only have the 415 nm. And so, is it ok if we use 415nm? would it effect the accuracy of the measurement significantly? Could give me the contract of distributor in Thailand. Thank you.

      Reply

      A

      adminSubmitted [ Apr 23 2019 ]

      Answered: Dear Thapanee, Thanks for your message. It is ok to use 412nm to test the kit. Pls contact with the distributor to have the kit. Pacific Science Co.,Ltd. 62,64 Soi Charansanitwong 49/1 Charansanitwong rd. Bangbumru Bangplad Bangkok 10700 THAILAND Tel : +662-4330068-9 ext. 212 Email : pacscien@ksc.th.com Any questions, pls feel free to ask. Best regards,

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