With the reduced coenzyme (NADPH) as hydrogen donor, oxidized glutathione (GSSG) can be reduced to reduced glutathione (GSH). The decrease of NADPH absorbance can be measured at 340 nm. The activity of GR can be calculated by detecting the change of NADPH.

Performance characteristics

Synonyms	GR
Sample type	serum;plasma;tissue;cell
Sensitivity	1.87 U/L
Detection range	1.87-150 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	50 min
Precision	Average inter-assay CV: 2.1-6.8%Average intra-assay CV: 1.5-3.9%
Other instruments required	Incubator, Vortex mixer, Micropipettor, Centrifuge, Magnetic stirrer
Other reagents required	Double distilled water, Normal saline (0.9% NaCl)，Deionized water
Storage	Reagent 1:2-8 ℃;Other reagent:-20 ℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K097-M	Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
E-BC-K030-M	Reduced Glutathione (GSH) Colorimetric Assay Kit
E-BC-K096-M	Glutathione Peroxidase (GSH-Px) Activity Assay Kit
E-BC-K025-M	Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

Cat. No	E-BC-K099-M
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha