Human 5-LO (Arachidonate 5-Lipoxygenase) CLIA Kit (E-CL-H0197)

Name: Human 5-LO (Arachidonate 5-Lipoxygenase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0197
Price: 180.0 USD
Availability: InStock

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Human 5-LO (Arachidonate 5-Lipoxygenase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human 5-LO in samples.No significant cross-reactivity or interference between Human 5-LO and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	56540.000	68590.000	62565.000	62533
2000	28206.000	28238.000	28222.000	28190
1000	15493.000	13463.000	14478.000	14446
500	7149.000	7327.000	7238.000	7206
250	3659.000	3157.000	3408.000	3376
125	1852.000	1624.000	1738.000	1706
62.5	807.000	853.000	830.000	798
0	32.000	32.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	211.920	560.500	1338.770	205.800	603.970	1412.790
Standard deviation	24.220	60.370	82.600	18.520	50.910	127.570
CV（%）	11.430	10.770	6.170	9.000	8.430	9.030

Recovery

The recovery of Human 5-LO was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-98	91
EDTA plasma （n=8）	94-108	99
Cell culture media （n=8）	88-100	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human 5-LO and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-113	95-111	95-110
1:2	Average	104	102	102
1:4	Range	99-117	93-108	100-120
1:4	Average	107	100	109
1:8	Range	98-111	91-102	101-115
1:8	Average	105	97	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 5-LO. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 5-LO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 5-LO, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human 5-LO. You can calculate the concentration of Human 5-LO in the samples by comparing the RLU value of the samples to the standard curve.