Human 5-NT (5-Nucleotidase) CLIA Kit (E-CL-H0198)

Name: Human 5-NT (5-Nucleotidase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0198
Price: 180.0 USD
Availability: InStock

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Human 5-NT (5-Nucleotidase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human 5-NT in samples.No significant cross-reactivity or interference between Human 5-NT and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	59080.000	66704.000	62892.000	62856
2000	26955.000	32197.000	29576.000	29540
1000	14991.000	13007.000	13999.000	13963
500	6370.000	7558.000	6964.000	6928
250	3665.000	3263.000	3464.000	3428
125	1731.000	1679.000	1705.000	1669
62.5	724.000	802.000	763.000	727
0	35.000	37.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	199.210	595.700	1640.790	210.420	621.130	1523.540
Standard deviation	16.260	48.550	179.990	18.290	52.730	155.400
CV（%）	8.160	8.150	10.970	8.690	8.490	10.200

Recovery

The recovery of Human 5-NT was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-103	95
EDTA plasma （n=8）	92-107	98
Cell culture media （n=8）	85-97	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human 5-NT and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-97	86-98	88-98
1:2	Average	91	92	93
1:4	Range	100-120	101-114	92-106
1:4	Average	109	107	100
1:8	Range	83-99	83-95	96-110
1:8	Average	90	90	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human 5-NT. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human 5-NT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human 5-NT, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human 5-NT. You can calculate the concentration of Human 5-NT in the samples by comparing the RLU value of the samples to the standard curve.

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