Human ABCA1(ATP-binding cassette transporter A1) CLIA Kit (E-CL-H0424)

Name: Human ABCA1(ATP-binding cassette transporter A1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0424
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ABCA1(ATP-binding cassette transporter A1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ABCA1 in samples.No significant cross-reactivity or interference between Human ABCA1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55004.000	65630.000	60317.000	60283
1000	23807.000	24895.000	24351.000	24317
500	12019.000	10539.000	11279.000	11245
250	5648.000	5782.000	5715.000	5681
125	2973.000	2735.000	2854.000	2820
62.5	1390.000	1356.000	1373.000	1339
31.25	653.000	705.000	679.000	645
0	33.000	35.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	95.550	293.750	912.590	99.940	276.530	980.130
Standard deviation	11.760	31.490	100.750	11.550	32.110	79.390
CV（%）	12.310	10.720	11.040	11.560	11.610	8.100

Recovery

The recovery of Human ABCA1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-116	106
EDTA plasma （n=8）	94-106	100
Cell culture media （n=8）	87-102	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ABCA1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-119	93-111	91-103
1:2	Average	109	101	97
1:4	Range	91-105	97-109	100-114
1:4	Average	98	103	106
1:8	Range	97-110	88-99	89-102
1:8	Average	104	94	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ABCA1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ABCA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ABCA1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ABCA1. You can calculate the concentration of Human ABCA1 in the samples by comparing the RLU value of the samples to the standard curve.

Background