Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) CLIA Kit (E-CL-H0132)

Name: Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0132
Price: 180.0 USD
Availability: InStock

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Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ACAT2 in samples.No significant cross-reactivity or interference between Human ACAT2 and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	53477.000	63333.000	58405.000	58371
1000	22889.000	26437.000	24663.000	24629
500	12230.000	11404.000	11817.000	11783
250	5212.000	5894.000	5553.000	5519
125	2920.000	2800.000	2860.000	2826
62.5	1412.000	1230.000	1321.000	1287
31.25	697.000	703.000	700.000	666
0	32.000	36.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	100.820	247.800	857.080	110.640	261.220	811.870
Standard deviation	11.400	27.360	61.020	11.210	24.950	59.590
CV（%）	11.310	11.040	7.120	10.130	9.550	7.340

Recovery

The recovery of Human ACAT2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-110	105
EDTA plasma （n=8）	89-103	95
Cell culture media （n=8）	92-107	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ACAT2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-107	85-99	86-99
1:2	Average	99	91	92
1:4	Range	88-102	86-98	96-110
1:4	Average	96	92	103
1:8	Range	88-102	88-104	91-105
1:8	Average	95	96	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACAT2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACAT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACAT2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACAT2. You can calculate the concentration of Human ACAT2 in the samples by comparing the RLU value of the samples to the standard curve.