Human ACE2 (Angiotensin I Converting Enzyme 2) CLIA Kit (E-CL-H0233)

Name: Human ACE2 (Angiotensin I Converting Enzyme 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0233
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ACE2 (Angiotensin I Converting Enzyme 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ACE2 in samples.No significant cross-reactivity or interference between Human ACE2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	60677.000	71849.000	66263.000	66229
2500	33721.000	35865.000	34793.000	34759
1250	20670.000	19202.000	19936.000	19902
625	10025.000	10343.000	10184.000	10150
312.5	5244.000	4506.000	4875.000	4841
156.25	2722.000	2690.000	2706.000	2672
78.13	1394.000	1444.000	1419.000	1385
0	33.000	35.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	264.480	543.750	1692.470	286.760	519.370	1672.550
Standard deviation	23.700	59.210	149.950	31.080	37.130	192.180
CV（%）	8.960	10.890	8.860	10.840	7.150	11.490

Recovery

The recovery of Human ACE2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	101-114	108
EDTA plasma （n=8）	86-100	92
Cell culture media （n=8）	89-101	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ACE2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-105	87-101	92-103
1:2	Average	97	93	98
1:4	Range	101-116	85-99	100-114
1:4	Average	108	92	106
1:8	Range	98-116	92-104	96-108
1:8	Average	106	98	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACE2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACE2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACE2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACE2. You can calculate the concentration of Human ACE2 in the samples by comparing the RLU value of the samples to the standard curve.

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