Human ACHAP (Acetylcholinesterase Associated Protein) CLIA Kit (E-CL-H0281)

Name: Human ACHAP (Acetylcholinesterase Associated Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0281
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ACHAP (Acetylcholinesterase Associated Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ACHAP in samples.No significant cross-reactivity or interference between Human ACHAP and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	58017.000	61881.000	59949.000	59922
1000	23314.000	25780.000	24547.000	24520
500	11592.000	10696.000	11144.000	11117
250	5513.000	5941.000	5727.000	5700
125	2860.000	2490.000	2675.000	2648
62.5	1413.000	1297.000	1355.000	1328
31.25	683.000	687.000	685.000	658
0	27.000	27.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	98.700	227.530	788.630	91.870	236.170	861.670
Standard deviation	11.900	21.840	56.780	9.140	25.030	67.810
CV（%）	12.060	9.600	7.200	9.950	10.600	7.870

Recovery

The recovery of Human ACHAP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-106	97
EDTA plasma （n=8）	93-108	100
Cell culture media （n=8）	87-103	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ACHAP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-107	86-101	90-102
1:2	Average	99	93	97
1:4	Range	97-110	100-113	89-103
1:4	Average	104	107	95
1:8	Range	91-105	99-113	96-108
1:8	Average	99	107	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACHAP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACHAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACHAP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACHAP. You can calculate the concentration of Human ACHAP in the samples by comparing the RLU value of the samples to the standard curve.