Human AChE (Acetylcholinesterase) CLIA Kit (E-CL-H0210)

Name: Human AChE (Acetylcholinesterase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0210
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human AChE (Acetylcholinesterase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human AChE in samples.No significant cross-reactivity or interference between Human AChE and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	32201.000	39141.000	35671.000	35639
10	11285.000	13733.000	12509.000	12477
5	5792.000	5652.000	5722.000	5690
2.5	2582.000	2662.000	2622.000	2590
1.25	1443.000	1405.000	1424.000	1392
0.63	988.000	854.000	921.000	889
0.31	616.000	656.000	636.000	604
0	32.000	32.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.090	2.330	9.790	1.150	2.270	9.260
Standard deviation	0.120	0.250	0.930	0.110	0.160	1.060
CV（%）	11.010	10.730	9.500	9.570	7.050	11.450

Recovery

The recovery of Human AChE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-97	91
EDTA plasma （n=8）	94-107	101
Cell culture media （n=8）	85-96	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AChE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-108	86-96	103-119
1:2	Average	101	91	110
1:4	Range	93-108	93-105	96-108
1:4	Average	100	98	101
1:8	Range	102-118	100-115	99-115
1:8	Average	108	108	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AChE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AChE. You can calculate the concentration of Human AChE in the samples by comparing the RLU value of the samples to the standard curve.

Background