Human ACP5 (Tartrate Resistant Acid Phosphatase 5) CLIA Kit (E-CL-H0995)

Name: Human ACP5 (Tartrate Resistant Acid Phosphatase 5) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0995
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ACP5 (Tartrate Resistant Acid Phosphatase 5) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ACP5 in samples.No significant cross-reactivity or interference between Human ACP5 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	33765.000	35581.000	34673.000	34650
5	16584.000	17086.000	16835.000	16812
2.5	7899.000	7303.000	7601.000	7578
1.25	3627.000	4253.000	3940.000	3917
0.63	1942.000	1808.000	1875.000	1852
0.31	921.000	877.000	899.000	876
0.16	351.000	381.000	366.000	343
0	23.000	23.000	23.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.550	0.910	4.720	0.560	0.970	4.610
Standard deviation	0.070	0.100	0.510	0.060	0.110	0.390
CV（%）	12.730	10.990	10.810	10.710	11.340	8.460

Recovery

The recovery of Human ACP5 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-106	98
EDTA plasma （n=8）	90-105	96
Cell culture media （n=8）	89-102	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ACP5 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-103	100-113	90-101
1:2	Average	95	106	96
1:4	Range	94-105	101-116	99-115
1:4	Average	99	107	106
1:8	Range	96-108	90-105	88-102
1:8	Average	103	96	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACP5. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACP5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACP5, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACP5. You can calculate the concentration of Human ACP5 in the samples by comparing the RLU value of the samples to the standard curve.

Background