Human ACTN2 (Actinin Alpha 2) CLIA Kit (E-CL-H0214)

Name: Human ACTN2 (Actinin Alpha 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0214
Price: 180.0 USD
Availability: InStock

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Human ACTN2 (Actinin Alpha 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ACTN2 in samples.No significant cross-reactivity or interference between Human ACTN2 and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	60060.000	69404.000	64732.000	64699
2500	33593.000	40187.000	36890.000	36857
1250	20437.000	19001.000	19719.000	19686
625	9987.000	10503.000	10245.000	10212
312.5	5283.000	5019.000	5151.000	5118
156.25	2593.000	2429.000	2511.000	2478
78.13	1442.000	1490.000	1466.000	1433
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	255.150	495.460	1831.610	235.730	520.100	1693.410
Standard deviation	32.070	41.720	152.760	20.580	40.100	187.460
CV（%）	12.570	8.420	8.340	8.730	7.710	11.070

Recovery

The recovery of Human ACTN2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-111	104
EDTA plasma （n=8）	89-102	95
Cell culture media （n=8）	84-96	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ACTN2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-101	90-102	94-110
1:2	Average	93	97	101
1:4	Range	94-105	96-108	98-114
1:4	Average	99	102	105
1:8	Range	95-109	94-111	92-104
1:8	Average	103	101	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTN2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTN2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACTN2. You can calculate the concentration of Human ACTN2 in the samples by comparing the RLU value of the samples to the standard curve.

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