Human ACTα1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit (E-CL-H0217)

Name: Human ACTα1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0217
Price: 180.0 USD
Availability: InStock

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Human ACTα1 (Actin Alpha 1, Skeletal Muscle) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ACTα1 in samples.No significant cross-reactivity or interference between Human ACTα1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	61649.000	67363.000	64506.000	64473
2000	28064.000	28782.000	28423.000	28390
1000	15894.000	13574.000	14734.000	14701
500	6900.000	7382.000	7141.000	7108
250	3513.000	3101.000	3307.000	3274
125	1803.000	1551.000	1677.000	1644
62.5	776.000	782.000	779.000	746
0	33.000	33.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	217.160	354.100	1555.530	218.090	327.990	1586.240
Standard deviation	24.100	37.710	98.310	27.920	32.730	153.230
CV（%）	11.100	10.650	6.320	12.800	9.980	9.660

Recovery

The recovery of Human ACTα1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-114	106
EDTA plasma （n=8）	96-112	103
Cell culture media （n=8）	86-98	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ACTα1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-111	100-114	90-103
1:2	Average	102	108	97
1:4	Range	100-117	87-102	86-101
1:4	Average	108	93	92
1:8	Range	86-98	97-109	92-109
1:8	Average	93	102	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTα1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTα1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTα1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ACTα1. You can calculate the concentration of Human ACTα1 in the samples by comparing the RLU value of the samples to the standard curve.

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