Human ADA (Adenosine Deaminase) CLIA Kit (E-CL-H0218)

Name: Human ADA (Adenosine Deaminase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0218
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ADA (Adenosine Deaminase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADA in samples.No significant cross-reactivity or interference between Human ADA and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	61103.000	73147.000	67125.000	67096
2500	34061.000	35839.000	34950.000	34921
1250	20119.000	19787.000	19953.000	19924
625	9353.000	9551.000	9452.000	9423
312.5	5302.000	5220.000	5261.000	5232
156.25	2603.000	2471.000	2537.000	2508
78.13	1434.000	1492.000	1463.000	1434
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	270.820	642.640	2431.000	253.500	660.830	2416.510
Standard deviation	33.420	70.110	271.300	29.580	64.700	249.140
CV（%）	12.340	10.910	11.160	11.670	9.790	10.310

Recovery

The recovery of Human ADA was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-109	100
EDTA plasma （n=8）	101-114	107
Cell culture media （n=8）	96-110	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADA and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-99	90-100	89-101
1:2	Average	91	95	96
1:4	Range	92-104	91-104	99-113
1:4	Average	98	98	107
1:8	Range	94-109	93-106	93-106
1:8	Average	100	99	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADA. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADA, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADA. You can calculate the concentration of Human ADA in the samples by comparing the RLU value of the samples to the standard curve.