Human ADAM10 (A Disintegrin And Metalloprotease 10) CLIA Kit (E-CL-H0219)

Name: Human ADAM10 (A Disintegrin And Metalloprotease 10) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0219
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ADAM10 (A Disintegrin And Metalloprotease 10) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADAM10 in samples.No significant cross-reactivity or interference between Human ADAM10 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	52548.000	57612.000	55080.000	55058
500	21327.000	25569.000	23448.000	23426
250	11345.000	9813.000	10579.000	10557
125	4882.000	5024.000	4953.000	4931
62.5	2584.000	2442.000	2513.000	2491
31.25	1359.000	1235.000	1297.000	1275
15.63	728.000	744.000	736.000	714
0	21.000	23.000	22.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	52.750	132.960	367.030	57.500	136.900	386.750
Standard deviation	6.470	13.240	33.180	6.660	15.940	23.590
CV（%）	12.270	9.960	9.040	11.580	11.640	6.100

Recovery

The recovery of Human ADAM10 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-106	101
EDTA plasma （n=8）	91-107	97
Cell culture media （n=8）	92-106	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADAM10 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-111	84-98	102-119
1:2	Average	105	90	110
1:4	Range	101-115	103-117	100-115
1:4	Average	107	110	108
1:8	Range	96-109	87-100	96-111
1:8	Average	102	93	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADAM10. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADAM10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADAM10, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADAM10. You can calculate the concentration of Human ADAM10 in the samples by comparing the RLU value of the samples to the standard curve.

Background