Human ADCY1 (Adenylate Cyclase 1, Brain) CLIA Kit (E-CL-H0223)

Name: Human ADCY1 (Adenylate Cyclase 1, Brain) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0223
Price: 180.0 USD
Availability: InStock

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Human ADCY1 (Adenylate Cyclase 1, Brain) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADCY1 in samples.No significant cross-reactivity or interference between Human ADCY1 and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	32892.000	35000.000	33946.000	33919
5	14919.000	17367.000	16143.000	16116
2.5	8196.000	7930.000	8063.000	8036
1.25	3655.000	3777.000	3716.000	3689
0.63	1842.000	1764.000	1803.000	1776
0.31	887.000	825.000	856.000	829
0.16	367.000	379.000	373.000	346
0	27.000	27.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.550	1.090	3.730	0.500	1.090	3.830
Standard deviation	0.060	0.100	0.410	0.050	0.090	0.380
CV（%）	10.910	9.170	10.990	10.000	8.260	9.920

Recovery

The recovery of Human ADCY1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-101	95
EDTA plasma （n=8）	99-111	105
Cell culture media （n=8）	89-105	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADCY1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-105	85-98	86-98
1:2	Average	98	90	93
1:4	Range	90-107	96-114	93-107
1:4	Average	98	104	98
1:8	Range	87-97	91-104	97-114
1:8	Average	92	98	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADCY1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADCY1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADCY1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADCY1. You can calculate the concentration of Human ADCY1 in the samples by comparing the RLU value of the samples to the standard curve.