Human ADCY2 (Adenylate Cyclase 2) CLIA Kit (E-CL-H0171)

Name: Human ADCY2 (Adenylate Cyclase 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0171
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ADCY2 (Adenylate Cyclase 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADCY2 in samples.No significant cross-reactivity or interference between Human ADCY2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	58757.000	71105.000	64931.000	64898
2000	26518.000	31496.000	29007.000	28974
1000	14356.000	13110.000	13733.000	13700
500	6297.000	7255.000	6776.000	6743
250	3595.000	3507.000	3551.000	3518
125	1643.000	1613.000	1628.000	1595
62.5	728.000	836.000	782.000	749
0	33.000	33.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	209.810	458.430	1973.500	223.510	473.050	2068.300
Standard deviation	17.600	33.100	139.530	26.780	47.870	235.790
CV（%）	8.390	7.220	7.070	11.980	10.120	11.400

Recovery

The recovery of Human ADCY2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-100	91
EDTA plasma （n=8）	90-101	95
Cell culture media （n=8）	101-117	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADCY2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-108	88-101	95-109
1:2	Average	101	93	103
1:4	Range	102-115	87-99	95-108
1:4	Average	109	94	102
1:8	Range	102-115	100-111	85-98
1:8	Average	109	105	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADCY2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADCY2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADCY2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADCY2. You can calculate the concentration of Human ADCY2 in the samples by comparing the RLU value of the samples to the standard curve.