Human ADM (Adrenomedullin) CLIA Kit (E-CL-H0228)

Name: Human ADM (Adrenomedullin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0228
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADM in samples.No significant cross-reactivity or interference between Human ADM and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	52065.000	60677.000	56371.000	56342
400	22137.000	25087.000	23612.000	23583
200	10954.000	9754.000	10354.000	10325
100	4575.000	5109.000	4842.000	4813
50	2337.000	2161.000	2249.000	2220
25	1145.000	1097.000	1121.000	1092
12.5	519.000	521.000	520.000	491
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	43.820	84.720	300.610	47.630	80.100	314.320
Standard deviation	4.600	7.480	21.010	5.590	7.420	31.680
CV（%）	10.500	8.830	6.990	11.740	9.260	10.080

Recovery

The recovery of Human ADM was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-115	108
EDTA plasma （n=8）	86-101	92
Cell culture media （n=8）	90-104	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADM and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-116	98-111	91-106
1:2	Average	107	105	96
1:4	Range	87-98	92-105	97-109
1:4	Average	93	98	104
1:8	Range	100-117	87-102	96-112
1:8	Average	108	94	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADM. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADM, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADM. You can calculate the concentration of Human ADM in the samples by comparing the RLU value of the samples to the standard curve.

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