Human ADP/Acrp30 (Adiponectin) CLIA Kit (E-CL-H0004)

Name: Human ADP/Acrp30 (Adiponectin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0004
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ADP/Acrp30 (Adiponectin) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.47 ng/mL
Detection Range	0.78-50 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADP/Acrp30 in samples.No significant cross-reactivity or interference between Human ADP/Acrp30 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
50	37486.000	42442.000	39964.000	39933
25	14840.000	17766.000	16303.000	16272
12.5	7333.000	6859.000	7096.000	7065
6.25	3567.000	3691.000	3629.000	3598
3.13	1898.000	1802.000	1850.000	1819
1.56	1155.000	1089.000	1122.000	1091
0.78	715.000	765.000	740.000	709
0	31.000	31.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	2.350	4.950	24.810	2.500	5.300	25.210
Standard deviation	0.300	0.570	2.740	0.210	0.620	1.970
CV（%）	12.770	11.520	11.040	8.400	11.700	7.810

Recovery

The recovery of Human ADP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-109	100
EDTA plasma （n=8）	100-112	106
Cell culture media （n=8）	96-111	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-112	97-110	95-111
1:2	Average	106	102	103
1:4	Range	99-113	84-97	100-117
1:4	Average	104	91	108
1:8	Range	101-115	88-101	91-105
1:8	Average	107	93	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADP/Acrp30. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADP/Acrp30. You can calculate the concentration of Human ADP/Acrp30 in the samples by comparing the RLU value of the samples to the standard curve.

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