Human ADRA1A (Adrenergic Receptor alpha-1A ) CLIA Kit (E-CL-H0229)

Name: Human ADRA1A (Adrenergic Receptor alpha-1A ) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0229
Price: 180.0 USD
Availability: InStock

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Human ADRA1A (Adrenergic Receptor alpha-1A ) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ADRA1A in samples.No significant cross-reactivity or interference between Human ADRA1A and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	61625.000	62015.000	61820.000	61783
2000	28619.000	30085.000	29352.000	29315
1000	15008.000	13832.000	14420.000	14383
500	6958.000	7554.000	7256.000	7219
250	3456.000	3122.000	3289.000	3252
125	1788.000	1672.000	1730.000	1693
62.5	751.000	817.000	784.000	747
0	36.000	38.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	185.440	371.170	1994.500	194.540	337.460	2101.720
Standard deviation	16.580	33.520	220.390	19.420	30.240	217.740
CV（%）	8.940	9.030	11.050	9.980	8.960	10.360

Recovery

The recovery of Human ADRA1A was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-111	105
EDTA plasma （n=8）	88-100	95
Cell culture media （n=8）	98-114	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ADRA1A and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-100	85-95	101-113
1:2	Average	93	90	107
1:4	Range	90-106	96-113	97-112
1:4	Average	97	103	104
1:8	Range	88-100	96-110	89-100
1:8	Average	95	101	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADRA1A. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADRA1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADRA1A, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ADRA1A. You can calculate the concentration of Human ADRA1A in the samples by comparing the RLU value of the samples to the standard curve.

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