Human AGC (Aggrecan) CLIA Kit (E-CL-H0240)

Name: Human AGC (Aggrecan) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0240
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human AGC in samples.No significant cross-reactivity or interference between Human AGC and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	63480.000	63752.000	63616.000	63590
2000	27073.000	30531.000	28802.000	28776
1000	15563.000	13583.000	14573.000	14547
500	6234.000	6986.000	6610.000	6584
250	3701.000	3233.000	3467.000	3441
125	1715.000	1465.000	1590.000	1564
62.5	746.000	854.000	800.000	774
0	25.000	27.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	211.020	401.400	1442.710	209.360	371.690	1299.240
Standard deviation	25.980	37.610	148.020	20.160	44.080	87.180
CV（%）	12.310	9.370	10.260	9.630	11.860	6.710

Recovery

The recovery of Human AGC was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-98	91
EDTA plasma （n=8）	94-108	101
Cell culture media （n=8）	94-106	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AGC and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-114	100-112	99-113
1:2	Average	106	106	104
1:4	Range	94-105	88-100	96-109
1:4	Average	100	95	101
1:8	Range	96-111	101-116	101-117
1:8	Average	102	109	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AGC. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGC, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AGC. You can calculate the concentration of Human AGC in the samples by comparing the RLU value of the samples to the standard curve.

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