Human AICAR (AICA Riboside) CLIA Kit (E-CL-H0946)

Name: Human AICAR (AICA Riboside) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0946
Price: 180.0 USD
Availability: InStock

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Human AICAR (AICA Riboside) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human AICAR in samples.No significant cross-reactivity or interference between Human AICAR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	52179.000	55239.000	53709.000	53679
250	19599.000	23607.000	21603.000	21573
125	9955.000	9191.000	9573.000	9543
62.5	4553.000	5281.000	4917.000	4887
31.25	2482.000	2436.000	2459.000	2429
15.63	1383.000	1247.000	1315.000	1285
7.81	726.000	760.000	743.000	713
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	25.900	74.560	178.040	27.750	77.390	184.350
Standard deviation	3.010	8.690	18.340	2.720	8.610	20.980
CV（%）	11.620	11.660	10.300	9.800	11.130	11.380

Recovery

The recovery of Human AICAR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-107	101
EDTA plasma （n=8）	94-106	99
Cell culture media （n=8）	92-105	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AICAR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-112	89-99	90-102
1:2	Average	106	94	95
1:4	Range	98-113	89-105	95-106
1:4	Average	106	96	100
1:8	Range	97-112	98-112	101-115
1:8	Average	102	104	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AICAR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AICAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AICAR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AICAR. You can calculate the concentration of Human AICAR in the samples by comparing the RLU value of the samples to the standard curve.