Human AIF1 (Allograft Inflammatory Factor 1) CLIA Kit (E-CL-H0246)

Name: Human AIF1 (Allograft Inflammatory Factor 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0246
Price: 180.0 USD
Availability: InStock

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Human AIF1 (Allograft Inflammatory Factor 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human AIF1 in samples.No significant cross-reactivity or interference between Human AIF1 and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	54515.000	55903.000	55209.000	55183
400	21441.000	24379.000	22910.000	22884
200	10143.000	9275.000	9709.000	9683
100	4658.000	4728.000	4693.000	4667
50	2532.000	2166.000	2349.000	2323
25	1138.000	1038.000	1088.000	1062
12.5	546.000	556.000	551.000	525
0	26.000	26.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	37.450	82.230	389.360	40.070	85.450	384.980
Standard deviation	4.360	6.460	40.420	3.940	7.580	38.840
CV（%）	11.640	7.860	10.380	9.830	8.870	10.090

Recovery

The recovery of Human AIF1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-102	95
EDTA plasma （n=8）	85-100	92
Cell culture media （n=8）	101-115	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AIF1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	84-96	86-99	99-111
1:2	Average	91	91	105
1:4	Range	90-101	100-114	88-101
1:4	Average	96	107	94
1:8	Range	93-105	85-97	94-108
1:8	Average	100	90	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AIF1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIF1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AIF1. You can calculate the concentration of Human AIF1 in the samples by comparing the RLU value of the samples to the standard curve.

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