Human AIF (Apoptosis Inducing Factor) CLIA Kit (E-CL-H0391)

Name: Human AIF (Apoptosis Inducing Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0391
Price: 180.0 USD
Availability: InStock

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Human AIF (Apoptosis Inducing Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human AIF in samples.No significant cross-reactivity or interference between Human AIF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	59030.000	62030.000	60530.000	60504
1000	24119.000	28407.000	26263.000	26237
500	12729.000	11257.000	11993.000	11967
250	5396.000	5406.000	5401.000	5375
125	2906.000	2810.000	2858.000	2832
62.5	1480.000	1310.000	1395.000	1369
31.25	706.000	754.000	730.000	704
0	25.000	27.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	95.600	250.840	948.150	102.820	227.580	911.560
Standard deviation	9.390	18.440	89.600	12.530	25.170	55.700
CV（%）	9.820	7.350	9.450	12.190	11.060	6.110

Recovery

The recovery of Human AIF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-100	92
EDTA plasma （n=8）	93-104	98
Cell culture media （n=8）	85-97	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AIF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-106	94-109	100-118
1:2	Average	101	100	108
1:4	Range	96-109	86-100	88-99
1:4	Average	103	93	94
1:8	Range	99-114	91-106	88-100
1:8	Average	105	99	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AIF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AIF. You can calculate the concentration of Human AIF in the samples by comparing the RLU value of the samples to the standard curve.

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