Human AIM2 (Absent In Melanoma 2) CLIA Kit (E-CL-H1428)

Name: Human AIM2 (Absent In Melanoma 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1428
Price: 180.0 USD
Availability: InStock

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Human AIM2 (Absent In Melanoma 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human AIM2 in samples.No significant cross-reactivity or interference between Human AIM2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56727.000	61349.000	59038.000	59007
1000	22822.000	27540.000	25181.000	25150
500	12311.000	11251.000	11781.000	11750
250	5307.000	5951.000	5629.000	5598
125	2767.000	2557.000	2662.000	2631
62.5	1464.000	1352.000	1408.000	1377
31.25	653.000	741.000	697.000	666
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	101.660	274.890	873.900	101.330	285.390	826.310
Standard deviation	8.370	29.410	57.420	10.800	28.620	65.610
CV（%）	8.230	10.700	6.570	10.660	10.030	7.940

Recovery

The recovery of Human AIM2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-99	94
EDTA plasma （n=8）	102-115	108
Cell culture media （n=8）	92-106	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AIM2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-107	88-102	93-108
1:2	Average	99	93	100
1:4	Range	87-98	86-98	93-107
1:4	Average	92	91	101
1:8	Range	100-114	94-104	84-100
1:8	Average	108	99	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AIM2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIM2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AIM2. You can calculate the concentration of Human AIM2 in the samples by comparing the RLU value of the samples to the standard curve.

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