Human ALDH(Aldehyde Dehydrogenase, Mitochondrial) CLIA Kit (E-CL-H0249)

Name: Human ALDH(Aldehyde Dehydrogenase, Mitochondrial) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0249
Price: 180.0 USD
Availability: InStock

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Human ALDH(Aldehyde Dehydrogenase, Mitochondrial) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ALDM in samples.No significant cross-reactivity or interference between Human ALDM and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	36318.000	34330.000	35324.000	35230
2500	16432.000	17214.000	16823.000	16729
1250	7460.000	7910.000	7685.000	7591
625	3601.000	3613.000	3607.000	3513
312.5	1916.000	1874.000	1895.000	1801
156.25	809.000	837.000	823.000	729
78.13	347.000	371.000	359.000	265
0	103.000	85.000	94.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	218.590	564.650	2070.200	243.230	530.280	2180.020
Standard deviation	13.590	25.420	113.340	11.980	24.870	123.110
CV（%）	5.390	4.920	5.130	5.040	4.950	5.220

Recovery

The recovery of Human ALDM was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-100	92
EDTA plasma （n=8）	86-96	90
Cell culture media （n=8）	97-108	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ALDM and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-109	98-111	96-107
1:2	Average	101	104	100
1:4	Range	95-106	96-112	99-110
1:4	Average	103	104	103
1:8	Range	87-97	92-105	95-107
1:8	Average	91	98	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ALDM. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALDM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALDM, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ALDM. You can calculate the concentration of Human ALDM in the samples by comparing the RLU value of the samples to the standard curve.