Human ALDOA (Aldolase A, Fructose Bisphosphate) CLIA Kit (E-CL-H0250)

Name: Human ALDOA (Aldolase A, Fructose Bisphosphate) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0250
Price: 180.0 USD
Availability: InStock

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Human ALDOA (Aldolase A, Fructose Bisphosphate) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ALDOA in samples.No significant cross-reactivity or interference between Human ALDOA and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	54122.000	62362.000	58242.000	58212
500	23117.000	24755.000	23936.000	23906
250	11046.000	9390.000	10218.000	10188
125	4936.000	5130.000	5033.000	5003
62.5	2729.000	2523.000	2626.000	2596
31.25	1386.000	1300.000	1343.000	1313
15.63	774.000	778.000	776.000	746
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	53.210	146.440	357.420	53.660	159.560	364.820
Standard deviation	6.120	12.650	38.850	6.260	17.520	32.100
CV（%）	11.500	8.640	10.870	11.670	10.980	8.800

Recovery

The recovery of Human ALDOA was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-108	102
EDTA plasma （n=8）	94-109	100
Cell culture media （n=8）	97-115	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ALDOA and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-105	92-109	90-102
1:2	Average	97	100	95
1:4	Range	84-99	103-116	102-118
1:4	Average	91	109	109
1:8	Range	87-102	85-97	92-108
1:8	Average	94	91	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ALDOA. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALDOA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALDOA, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ALDOA. You can calculate the concentration of Human ALDOA in the samples by comparing the RLU value of the samples to the standard curve.

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