Human ALDOC (Aldolase C, Fructose Bisphosphate) CLIA Kit (E-CL-H0893)

Name: Human ALDOC (Aldolase C, Fructose Bisphosphate) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0893
Price: 180.0 USD
Availability: InStock

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Human ALDOC (Aldolase C, Fructose Bisphosphate) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ALDOC in samples.No significant cross-reactivity or interference between Human ALDOC and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	33002.000	33794.000	33398.000	33370
5	15942.000	16714.000	16328.000	16300
2.5	8474.000	7200.000	7837.000	7809
1.25	3747.000	3831.000	3789.000	3761
0.63	1917.000	1675.000	1796.000	1768
0.31	943.000	849.000	896.000	868
0.16	359.000	365.000	362.000	334
0	27.000	29.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.490	1.190	4.960	0.490	1.180	4.790
Standard deviation	0.040	0.110	0.420	0.060	0.130	0.400
CV（%）	8.160	9.240	8.470	12.240	11.020	8.350

Recovery

The recovery of Human ALDOC was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-103	94
EDTA plasma （n=8）	95-111	103
Cell culture media （n=8）	92-106	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ALDOC and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-110	98-112	99-111
1:2	Average	104	105	105
1:4	Range	90-105	89-99	102-117
1:4	Average	97	94	109
1:8	Range	97-114	100-115	96-107
1:8	Average	105	107	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ALDOC. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALDOC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALDOC, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ALDOC. You can calculate the concentration of Human ALDOC in the samples by comparing the RLU value of the samples to the standard curve.