Human AMY1 (Amylase Alpha 1, Salivary) CLIA Kit (E-CL-H0258)

Name: Human AMY1 (Amylase Alpha 1, Salivary) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0258
Price: 180.0 USD
Availability: InStock

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Human AMY1 (Amylase Alpha 1, Salivary) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human AMY1 in samples.No significant cross-reactivity or interference between Human AMY1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	36064.000	37248.000	36656.000	36617
10	13018.000	13212.000	13115.000	13076
5	5380.000	5166.000	5273.000	5234
2.5	2583.000	2805.000	2694.000	2655
1.25	1482.000	1448.000	1465.000	1426
0.63	941.000	847.000	894.000	855
0.31	640.000	664.000	652.000	613
0	38.000	40.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.920	1.760	8.220	0.970	1.830	8.710
Standard deviation	0.100	0.130	0.550	0.080	0.140	0.540
CV（%）	10.870	7.390	6.690	8.250	7.650	6.200

Recovery

The recovery of Human AMY1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-98	92
EDTA plasma （n=8）	95-111	103
Cell culture media （n=8）	87-98	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AMY1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-108	96-108	91-104
1:2	Average	99	102	97
1:4	Range	88-98	95-108	89-103
1:4	Average	93	102	96
1:8	Range	97-111	90-105	92-108
1:8	Average	102	97	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AMY1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AMY1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AMY1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AMY1. You can calculate the concentration of Human AMY1 in the samples by comparing the RLU value of the samples to the standard curve.

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