Human ANG-R-Tie1 (Angiopoietin Receptor Tie1) CLIA Kit (E-CL-H0272)

Name: Human ANG-R-Tie1 (Angiopoietin Receptor Tie1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0272
Price: 180.0 USD
Availability: InStock

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Human ANG-R-Tie1 (Angiopoietin Receptor Tie1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ANG-R-Tie1 in samples.No significant cross-reactivity or interference between Human ANG-R-Tie1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57816.000	59762.000	58789.000	58760
1000	24699.000	27929.000	26314.000	26285
500	11414.000	10820.000	11117.000	11088
250	5367.000	6171.000	5769.000	5740
125	2974.000	2752.000	2863.000	2834
62.5	1396.000	1286.000	1341.000	1312
31.25	702.000	706.000	704.000	675
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	104.690	189.380	745.080	102.310	176.920	678.080
Standard deviation	11.710	17.670	78.680	12.500	17.340	65.030
CV（%）	11.190	9.330	10.560	12.220	9.800	9.590

Recovery

The recovery of Human ANG-R-Tie1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-112	102
EDTA plasma （n=8）	88-102	94
Cell culture media （n=8）	94-111	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ANG-R-Tie1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-102	88-99	86-99
1:2	Average	96	94	91
1:4	Range	96-109	101-116	90-101
1:4	Average	102	107	96
1:8	Range	85-97	101-117	87-100
1:8	Average	91	110	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG-R-Tie1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG-R-Tie1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANG-R-Tie1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ANG-R-Tie1. You can calculate the concentration of Human ANG-R-Tie1 in the samples by comparing the RLU value of the samples to the standard curve.

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