Human ANGPTL4 (Angiopoietin Like Protein 4) CLIA Kit (E-CL-H0270)

Name: Human ANGPTL4 (Angiopoietin Like Protein 4) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0270
Price: 180.0 USD
Availability: InStock

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Human ANGPTL4 (Angiopoietin Like Protein 4) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ANGPTL4 in samples.No significant cross-reactivity or interference between Human ANGPTL4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	33610.000	34862.000	34236.000	34206
10	12550.000	14654.000	13602.000	13572
5	5457.000	5087.000	5272.000	5242
2.5	2444.000	2808.000	2626.000	2596
1.25	1476.000	1318.000	1397.000	1367
0.63	999.000	895.000	947.000	917
0.31	664.000	668.000	666.000	636
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.100	1.960	7.250	0.990	2.010	7.600
Standard deviation	0.140	0.230	0.680	0.110	0.180	0.490
CV（%）	12.730	11.730	9.380	11.110	8.960	6.450

Recovery

The recovery of Human ANGPTL4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-107	99
EDTA plasma （n=8）	88-99	93
Cell culture media （n=8）	95-108	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ANGPTL4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-112	86-98	87-101
1:2	Average	102	91	92
1:4	Range	87-99	89-99	99-115
1:4	Average	93	94	106
1:8	Range	94-111	87-101	95-110
1:8	Average	101	93	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANGPTL4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANGPTL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ANGPTL4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ANGPTL4. You can calculate the concentration of Human ANGPTL4 in the samples by comparing the RLU value of the samples to the standard curve.

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