Human APAF1 (Apoptosis Protease Activating Factor 1) CLIA Kit (E-CL-H0393)

Name: Human APAF1 (Apoptosis Protease Activating Factor 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0393
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human APAF1 (Apoptosis Protease Activating Factor 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human APAF1 in samples.No significant cross-reactivity or interference between Human APAF1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56853.000	59581.000	58217.000	58175
1000	24321.000	24857.000	24589.000	24547
500	11899.000	10741.000	11320.000	11278
250	5449.000	6173.000	5811.000	5769
125	2779.000	2741.000	2760.000	2718
62.5	1383.000	1295.000	1339.000	1297
31.25	673.000	751.000	712.000	670
0	41.000	43.000	42.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	101.830	181.960	794.970	111.670	168.580	720.890
Standard deviation	12.170	15.080	89.590	13.090	17.110	68.990
CV（%）	11.950	8.290	11.270	11.720	10.150	9.570

Recovery

The recovery of Human APAF1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-109	103
EDTA plasma （n=8）	91-106	98
Cell culture media （n=8）	84-97	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human APAF1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-107	88-102	101-115
1:2	Average	97	93	109
1:4	Range	100-117	98-113	94-106
1:4	Average	107	105	101
1:8	Range	96-112	85-95	91-105
1:8	Average	102	90	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human APAF1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human APAF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human APAF1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human APAF1. You can calculate the concentration of Human APAF1 in the samples by comparing the RLU value of the samples to the standard curve.