Human APP (Amyloid Precursor Protein) CLIA Kit (E-CL-H0794)

Name: Human APP (Amyloid Precursor Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0794
Price: 180.0 USD
Availability: InStock

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Human APP (Amyloid Precursor Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human APP in samples.No significant cross-reactivity or interference between Human APP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	53937.000	57373.000	55655.000	55621
500	22047.000	24191.000	23119.000	23085
250	11201.000	9805.000	10503.000	10469
125	4568.000	5270.000	4919.000	4885
62.5	2686.000	2644.000	2665.000	2631
31.25	1405.000	1329.000	1367.000	1333
15.63	691.000	725.000	708.000	674
0	33.000	35.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	48.420	135.330	437.240	47.860	129.700	438.180
Standard deviation	5.780	11.260	42.410	5.070	11.180	40.400
CV（%）	11.940	8.320	9.700	10.590	8.620	9.220

Recovery

The recovery of Human APP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-103	97
EDTA plasma （n=8）	88-102	93
Cell culture media （n=8）	84-96	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human APP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-108	92-106	94-108
1:2	Average	100	98	100
1:4	Range	87-99	89-103	87-98
1:4	Average	94	95	93
1:8	Range	87-98	97-113	92-108
1:8	Average	92	104	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human APP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human APP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human APP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human APP. You can calculate the concentration of Human APP in the samples by comparing the RLU value of the samples to the standard curve.

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